Browsing Dissertations and Theses by Author "Chemistry"
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Development of Green Subcritical Water Separation Technology
Doctor, Ninad (East Carolina University, 2019-08-19)Theoretically speaking, water under temperature and pressure conditions below its critical point is called subcritical water. However, the term “subcritical water” normally refers to hot water up to 374 C and pressure up ... -
Development of NMR Methodologies to Study Site Specific Zinc-Protein Interactions
Benton, Amy Musgrave (East Carolina University, 2021-07-26)Found in all three biological domains of life and the second most abundant metal in the human body, zinc (Zn2+) is essential to various cellular processes like the metabolism of DNA and RNA, gene expression, and the ... -
DEVELOPMENT OF UNNATURAL SUBSTRATES AND TRYPTOPHAN AMINO ACIDS TO STUDY PROTON COUPLED ELECTRON TRANSFER IN ENZYMES
Ohgo, Kei (East Carolina University, 2020-12-09)Proton-coupled electron transfer plays an important role in substrate oxidation by C-H bond cleavage and long-range pathways associated with bioenergetics. This thesis is focused on the synthesis of unnatural substrates ... -
DIFFERENTIATION OF J-SERIES PROSTAGLANDINS AS PRO-APOPTOTIC PRODUCTS OF CANCER CELL METABOLISM
Kobet, Robert A. (East Carolina University, 2016-12-16)Cyclopentenone J-series prostaglandins and prostaglandin-ethanolamide conjugates have been shown to selectively induce endoplasmic reticulum stress-apoptosis in different cancer cell types. These lipids have been identified ... -
DIRECT ELECTROCHEMICAL ANALYSIS OF THE REDOX ACTIVITY OF TRYPTOPHAN AND TYROSINE IN MODIFIED AZURINS: THE IMPACT OF THE PROTEIN ENVIRONMENT
Tyson, Kris Janell (East Carolina University, 2021-05-03)Proton-coupled electron transfer (PCET) is a biological process essential to life. It is imperative for respiration in animals as well as photosynthesis in plants. Long-range PCET is often facilitated by redox-active amino ... -
Electrochemical Detection of Anti-Biofilm Activity Using Unnatural Amino Acid-Containing Antimicrobial Peptides
Vinogradov, Sergey M. (East Carolina University, 2015)Bacterial infections are a significant health problem that can be detrimental to the human population. It is estimated that bacterial infections are responsible for billions of dollars’ worth of damages in the health care ... -
ELECTROCHEMICAL DETECTION OF BENZO[A]PYRENE METABOLITE DNA DAMAGE : IMPLICATIONS OF NUCLEOBASE SEQUENCE AND ADDUCT STEREOCHEMISTRY
Satterwhite, Jennifer Elizabeth (East Carolina University, 2011)Xenobiotics are chemical compounds introduced to living organisms that originate outside the body. A well-studied xenobiotic is benzo[a]pyrene (BP), a polyaromatic hydrocarbon (PAH) primarily introduced into the body via ... -
Electrochemical Detection of Benzo[a]Pyrene-Induced DNA Damage at TP53 Oligomers : Impact of 5'-Methyl Cytosine and Bioactivation on the Genotoxicity Process
Trumbo, Caitlin M. (East Carolina University, 2014)DNA houses the blueprint that dictates how an organism will develop. However, DNA features numerous reactive sites that can be attacked by chemicals and radiation, resulting in DNA damage and possibly mutations. Chemical ... -
ELECTROSPRAY IONIZATION TIME OF FLIGHT MASS SPECTROMETRY OF POLY(METHYL METHACRYLATE) AND POLYSTYRENE USING SURFACE ACTIVE QUATERNARY AMMONIUM SALTS
Williams, Christopher M. (East Carolina University, 2011)Electrospray ionization time of flight mass spectrometry (ESI-ToF-MS) is a powerful characterization technique for large nonvolatile compounds, but has found limited applicability in the synthetic polymer field. Two factors ... -
Elucidating "Consumption" : Using Fluorescent Steroid Probes to Understand Host Cholesterol Utilization by Mycobacterium spp.
Wercholuk, Ashley N. (East Carolina University, 2014)Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB) disease, is the leading cause of death due to bacterial infection worldwide, claiming nearly two million lives each year. Often, the immune system ... -
Elucidating the Mechanism of Action of Unnatural Amino Acid Containing Antimicrobial Peptides in Membrane Environments
Clark, Tiffany D. (East Carolina University, 2012)Organism resistance continues to develop to the currently available antimicrobial compounds necessitating the development of innovative new therapeutic compounds with different specificities and mechanisms of action that ... -
Elucidating the Mechanisms of Transthyretin Aggregation in Transthyretin Amyloidosis
Dasari, Anvesh K. R. (East Carolina University, 2018-07-24)Accumulation of insoluble aggregates (amyloids) is a characteristic feature of many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. Aggregation and deposition of Transthyretin (TTR) protein ... -
EXPLORATION INTO THE SCOPE AND MECHANISM OF THE PLATINUM-CATALYZED ACYLATION OF 2-(ARYLOXY)PYRIDINES
Guthrie, Jacob D (East Carolina University, 2019-07-11)Transition metal catalysts have played a key role in direct C-H bond functionalization. However, one main drawback of these reactions is that oxidants and additives are often required to regenerate the active catalyst, ... -
EXPLORING STUDENT EXPERIENCES ACROSS A LINKED SEQUENCE COURSE-BASED UNDERGRADUATE RESEARCH EXPERIENCE
Whiting, Brandon (East Carolina University, 2020-11-23)This study reports the effect a linked Course-based Undergraduate Research Experience (CURE) has on a students' understanding of research and the associated experiences they encountered. CUREs offer an alternative path to ... -
Fluorescent Derivatives of Prostamides: Tools for Studying Anti-Cancer Activity
Stanley, Jordan Lynne (East Carolina University, 2018-07-23)The endocannabinoid arachidonoyl ethanolamide (AEA) and prostaglandins derived from it selectively induce apoptosis in tumorigenic cell lines due to overexpression of COX-2. It is likely that a novel J-series prostamide, ... -
Fundamental and Applied Studies on Chromatographic Separation of Cold Drugs and Skincare Creams and Extraction of Salvia miltiorrhiza using Subcritical Water
Kapalavavi, Brahmam (East Carolina University, 2014)Subcritical water chromatography (SBWC) and subcritical water extraction (SBWE) are two green techniques that use subcritical water as the sole solvent for separations, thus eliminating the use of toxic and expensive organic ... -
HOW CHARGED RESIDUES INFLUENCE THE THERMAL STABILITY OF COLLAGEN: A STUDY WITH NATURAL AND NON-NATURAL AMINO ACIDS
Banzon, Patrick D. (East Carolina University, 2020-09-16)Triple-helical collagens are key structural proteins in mammals. Their ubiquity and diverse functions drive our interest into understanding their behavior at a fundamental level. This thesis describes a reductionist approach ... -
IDENTIFICATION OF NOVEL CYTOTOXIC PROSTAGLANDIN METABOLITES PRODUCED IN ARACHIDONOYL ETHANOLAMIDE-TREATED TUMOREGENIC KERATINOCYTES
Thati, Drisheka (East Carolina University, 2012)Arachidonoyl ethanolamide (AEA) induces apoptosis in mouse tumorigenic keratinocytes (JWF-2 cells). Our previous data show that AEA is metabolized by COX-2 to pro-apoptotic J-series prostaglandins. COX-2 is an enzyme that ... -
Identifying compensatory genomic changes due to nickel (Ni2+) exposure: electrochemistry and quantifying DNA damage
Turner, Ryne (East Carolina University, 2020-11-24)Nickel is a toxic heavy metal that has been shown to cause adverse healthconditions in organisms. DNA damage has been observed in nematodes in response to nickel exposure. Nematodes originating from environments with high ... -
Improving Prostamide Anti-Cancer Activity Through Derivatization and Micellar Delivery
Halatek, David James (East Carolina University, 2023-04-28)Colorectal cancer is the fourth most common cancer diagnosis per year as well as the fourth highest rate of death per year according to the Centers for Disease Control. Approximately 1/3rd of the diagnosed colorectal cancer ...