Browsing Master's Theses by Author "Offenbacher, Adam R"
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Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases
Roberts, Daniella (East Carolina University, 2022-04-27)Lipoxygenases (LOXs) are a family of enzymes found in plants, animals, fungi, and bacteria that catalyze the per-oxidation of polyunsaturated fatty acids. In plants, LOXs are involved in growth, development, and defense ... -
Defining Protein Motions the Comprise the Reaction Barrier in Human Epithelial 15-Lipoxygenase-2
Ohler, Amanda (East Carolina University, 2022-04-27)Proteins are dynamic in nature, with these motions playing a role in substrate binding and product release. Protein thermal motions have emerged as participating in the bond making/breaking steps of catalysis and by extension ... -
Determination of the Role of Tyrosine-319 on the Efficiency of Photoinduced Radical Pairs in Avian Cryptochrome Photoreceptor Proteins Related to Magnetoreception
Signorelli, Rachel Lee (East Carolina University, 2023-07-17)Cryptochromes (CRYs) are flavin-containing blue light photoreceptors which are involved in circadian rhythms and magnetoreception. CRYs are structurally related to DNA photolyases. While photolyases use light-driven FAD ... -
DEVELOPMENT OF UNNATURAL SUBSTRATES AND TRYPTOPHAN AMINO ACIDS TO STUDY PROTON COUPLED ELECTRON TRANSFER IN ENZYMES
Ohgo, Kei (East Carolina University, 2020-12-09)Proton-coupled electron transfer plays an important role in substrate oxidation by C-H bond cleavage and long-range pathways associated with bioenergetics. This thesis is focused on the synthesis of unnatural substrates ... -
DIRECT ELECTROCHEMICAL ANALYSIS OF THE REDOX ACTIVITY OF TRYPTOPHAN AND TYROSINE IN MODIFIED AZURINS: THE IMPACT OF THE PROTEIN ENVIRONMENT
Tyson, Kris Janell (East Carolina University, 2021-05-03)Proton-coupled electron transfer (PCET) is a biological process essential to life. It is imperative for respiration in animals as well as photosynthesis in plants. Long-range PCET is often facilitated by redox-active amino ... -
Structure-Function Relationship of Surface N-glycans and Enzyme Active Site Proficiency: A Kinetic and Hydrogen-Deuterium Exchange Investigation of MoLOX
Whittington, Chris (East Carolina University, 2021-07-08)A 'unique' enzyme has emerged within the lipoxygenase (LOX) family. This manganese containing enzyme comes from the pathogen Magnaporthe oryzae and termed MoLOX. MoLOX is secreted at the onset of pathogenesis when the rice ... -
THE IMPACT OF GLYCOSYLATION ON ACTIVE SITE STRUCTURE AND ACTIVITY IN THE FUNGUS ENZYME MOLOX
Kostenko, Anastasiia (East Carolina University, 2019-05-01)Lipoxygenases from pathogenic fungi belong to the lipoxygenase family that catalyze the C-H activation of polyunsaturated fatty acids to form diverse hydroperoxides. While the lipoxygenase catalytic domains are structurally ...