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Now showing items 21-30 of 175
USING SEDIMENT ORGANIC GEOCHEMISTRY TO INTERPRET LATE HOLOCENE BARRIER ISLAND AND ESTUARINE EVOLUTION, NORTH CAROLINA, USA
(East Carolina University, 2014)
Deconvolving the relationship between meteorological and oceanographic phenomena and associated impacts to coastal systems is critical to understanding the future of coastal systems worldwide. North Carolina's barrier ...
Paternal diet and exercise epigenetically program energy expenditure and glucose metabolism in mouse offspring
(East Carolina University, 2014)
It is currently estimated that a third of Americans suffer from metabolic syndrome, which is an obesogenic disease shown to increase risk for developing type 2 diabetes (T2DM) by five-fold (Diabetes Care, 2012). The ...
EXPLORING THE PERCEPTIONS AND PRACTICES OF NURSES WORKING IN ADULT INTENSIVE CARE UNITS REGARDING CHILD VISITATION AND THE ROLE OF A CHILD LIFE SPECIALIST IN THIS SETTING
(East Carolina University, 2014)
There is little documented research within the United States regarding the issue of child visitation on adult intensive care units. Using an online cross-sectional survey, the current study explored perceptions and practices ...
Note taking skills using the LiveScribe pen: implications for college students diagnosed with learning disabilities
(East Carolina University, 2014)
The purpose of this study was to examine whether the use of strategic note-taking with the LiveScribe© pen increased the quantity of idea units, quality of notes written down, and retention of information provided for ...
Influence of Climatic Oscillations on Indian Ocean Tropical Cyclone Energy
(East Carolina University, 2014)
Tropical Cyclones form in 4 independent regions of the Indian Ocean Basin ranging from the Arabian Sea, Bay of Bengal, Western South Indian, and Eastern South Indian Ocean. These cyclones lead to distinct patterns in their ...
MAJOR AND TRACE ELEMENT CHEMICAL COMPOSITION OF GAHNITE FROM GRANITIC PEGMATITES AND A METAMORPHOSED MASSIVE SULFIDE DEPOSIT : SIGNIFICANCE FOR PEGMATITE FRACTIONATION AND DISCRIMINATION BETWEEN Li-RICH AND Li-POOR PEGMATITES
(East Carolina University, 2014)
Rare-element granitic pegmatites are common hosts to economic deposits of rare metals, including Li, Ta, and rare-earth elements, which are increasing in economic importance due to advancing technology. Gahnite (ZnAl₂O₄) ...
A Case of Extremest Necessity? : Baltimore and Habeas Corpus in April 1861.
(East Carolina University, 2014)
Executive war powers are embedded in the United States Constitution.The circumstances in which they are used take on an even greater importance when these war powers come in conflict with an individual's constitutionally ...
Modern Greece : Values of a Civil War Blockade-Runner
(East Carolina University, 2014-11-12)
On June 27, 1862, during the American Civil War, the blockade-runner Modern Greece ran aground off the coast of Wilmington, NC. The ship was within reach of the guns of Fort Fisher, protecting the vessel from the Union ...
Source of saltwater intrusion in the Castle Hayne aquifer on the western part of Bogue Banks barrier island, North Carolina
(East Carolina University, 2014)
Saltwater intrusion is occurring in the confined Castle Hayne aquifer on the western part of the east-west trending barrier island of Bogue Banks, North Carolina. Of eleven municipal wells that are solely screened in the ...
Expression of microRNA in Alveolar Macrophages Deficient in PPARy
(East Carolina University, 2014)
The nuclear transcription factor Peroxisome proliferator-activated receptor gamma (PPARgamma) is a negative regulator of macrophage activation and inflammatory mediators. Alveolar macrophages of healthy individuals constitutively express PPARgamma Decreased activity and expression of PPARgamma are observed in the alveolar macrophages from patients suffering from inflammatory conditions such as pulmonary alveolar proteinosis (PAP) and sarcoidosis. These finding suggest that PPARf× activity may have an integral role in maintaining lung homeostasis. This study tested the hypothesis that microRNA expression would be dysregulated in murine alveolar macrophages deficient in PPARgamma. microRNA (miR) are small non-coding RNA molecules that post-transcriptionally regulate the expression of messenger RNA. Evaluation of microRNA in the murine model of PAP, the GM-CSF-KO mouse, demonstrates the elevation of miR-27a and miR-27b which target PPARgamma. The deficiency of PPARgamma and the lipid transporters ABCA1 and ABCG1 have been shown to contribute to the pathology of PAP. The microRNA miR-33-3p and miR-33-5p, which target these lipid transporters, were also elevated in GM-CSF-KO mice. Pulmonary granulomas comparable to those observed in pulmonary sarcoidosis are induced by instillation of multiwall carbon nanotubes (MWCNT) in C57Bl/6 mice. These animals have decreased PPARgamma activity and show elevated expression of miR-27a and miR-27b. It was also observed that the expression of the transporters ABCA1 and ABCG1 were decreased in MWCNT instilled mice. Expression of miR-33-3p and miR-33-5p was elevated in MWCNT instilled animals. The expression of microRNA that affects the activity of NF-£eB is also elevated in both murine models. We next investigated the use of PPARgamma agonist rosiglitazone on the expression of microRNA and messenger RNA. The use of rosiglitazone altered the expression of microRNA in both GM-CSF-KO and C57Bl/6+MWCNT mice. Rosiglitazone treatment altered the expression of the lipid transporter ABCA1and ABCG1 in C57Bl/6+MWCNT mice. The elevation of proinflammatory cytokines was also observed. Taken together, these observations support the hypothesis that PPARgamma activity effects the microRNA and gene expression in alveolar macrophages which is critical to overall lung homeostasis. Understanding the relationship between PPARgamma and microRNA in alveolar macrophage biology will provide insight into the regulation of the lung environment and possible therapeutic targets. ...