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Installation of Metal-Binding Functionality at Lysine Residues via Azide-Alkyne "Click Chemistry"
(2015-04-29)
A novel non-natural amino acid designed to bind divalent metal ions, dubbed Lys(PYRIT), was prepared in a “click reaction” by treating Fmoc-Lys(N3)-OH with 2-ethynylpyridine and Cu(I). It was incorporated into a 12-residue ...
Electrochemical Detection of Anti-Biofilm Activity Using Unnatural Amino Acid-Containing Antimicrobial Peptides
(East Carolina University, 2015)
Bacterial infections are a significant health problem that can be detrimental to the human population. It is estimated that bacterial infections are responsible for billions of dollars’ worth of damages in the health care ...
Molecular Dynamics Studies of Point Mutations of Cardiac Troponin C and Annexin
(East Carolina University, 2015)
Calcium binding proteins are biologically important for their ability to convert changes in calcium ion concentration to functional changes in proteins. All calcium binding proteins use a calcium sensing motif that changes ...
Kinetic Modeling of Batch Reaction Processes
(East Carolina University, 2012)
Kinetic models have been demonstrated to be useful in on-line batch monitoring systems. The ability to monitor a reaction in real time is invaluable to the production process of industrial and pharmaceutical products. ...
Synthesis and Reactions of Novel Heteroarylzirconocene Reagents
(East Carolina University, 2012)
The cross-coupling of metallated heteroaromatic species with aryl and heteroaryl halides is a powerful and highly desirable transformation in both the synthesis of pharmaceutical compounds as well as materials development. ...
A Calorimetric and Spectroscopic Study of the Interactions Between Model Lipid Membrane Bilayers and Simple Sugars
(East Carolina University, 2015)
It is well known that various organisms, such as the tardigrade, naturally use carbohydrates, particularly disaccharides, to help preserve their cell structural and functional integrity during freezing or dehydration. There ...
KINETIC MODELING OF DISSOLUTION AND CRYSTALLIZATION OF BATCH REACTIONS WITH IN SITU SPECTROSCOPIC MEASUREMENTS
(East Carolina University, 2012)
The use of Process Analytical Technology (PAT) recommended by the Food and Drug Administration (FDA) has significantly increased during the past years in the design, control and monitoring of pharmaceutical or chemical ...
IDENTIFICATION OF NOVEL CYTOTOXIC PROSTAGLANDIN METABOLITES PRODUCED IN ARACHIDONOYL ETHANOLAMIDE-TREATED TUMOREGENIC KERATINOCYTES
(East Carolina University, 2012)
Arachidonoyl ethanolamide (AEA) induces apoptosis in mouse tumorigenic keratinocytes (JWF-2 cells). Our previous data show that AEA is metabolized by COX-2 to pro-apoptotic J-series prostaglandins. COX-2 is an enzyme that ...
Thermodynamic studies of Ca²⁺ binding to human cardiac troponin C
(East Carolina University, 2012)
Human Cardiac Troponin C (HcTnC) is an EF hand protein responsible for initiating contraction of the myocardium (heart muscle). The helix-loop-helix motif, characteristic of members of the EF-hand family, allows HcTnC to ...
Counting Protons: Using Isothermal Titration Calorimetry to Determine the Number of Protons Exchanged Upon Ca(II) and Cd(II) Binding to Wild-type HcTnC and Mutant C84A and C35A/C84A Proteins
(East Carolina University, 2018-07-20)
Maintaining a delicate balance of metal ions in the cell is crucial to the survival of many organisms. Divalent cadmium, Cd(II), is a non-essential metal ion that can disrupt this delicate balance and lead to deleterious ...