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Probing the Kinetic Anabolism of Poly-Beta-Hydroxybutyrate in Cupriavidus necator H16 Using Single-Cell Raman Spectroscopy
(2016-08)
Poly-beta-hydroxybutyrate (PHB) can be formed in large amounts in Cupriavidus necator and is important for the industrial production of biodegradable plastics. In this investigation, laser tweezers Raman spectroscopy (LTRS) ...
Evolution of plant nucleotide-sugar interconversion enzymes
(2011-11-18)
Nucleotide-diphospho-sugars (NDP-sugars) are the building blocks of diverse polysaccharides and glycoconjugates in all organisms. In plants, 11 families of NDP-sugar interconversion enzymes (NSEs) have been identified, ...
Genome-wide functional analysis of the cotton transcriptome by creating an integrated EST database
(2011-11-08)
A total of 28,432 unique contigs (25,371 in consensus contigs and 3,061 as singletons) were assembled from all 268,786 cotton ESTs currently available. Several in silico approaches [comparative genomics, Blast, Gene Ontology ...
Experimental design and statistical rigor in phylogenomics of horizontal and endosymbiotic gene transfer
(2011-09-16)
A growing number of phylogenomic investigations from diverse eukaryotes are examining conflicts among gene trees as evidence of horizontal gene transfer. If multiple foreign genes from the same eukaryotic lineage are found ...
Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression
(2011-04-06)
Proper coordination of the functions at the DNA replication fork is vital to the normal functioning of a cell. Specifically the precise coordination of helicase and polymerase activity is crucial for efficient passage ...
Executive summary: "Mantle Frontier" workshop
(2011-03)
The workshop on “Reaching the Mantle Frontier: Moho and Beyond” was held at the Broad Branch Road Campus of the Carnegie Institution of Washington on 9–11 September 2010. The workshop attracted seventy-four scientists and ...
The Impact of Locoregional Therapy in Nonmetastatic Inflammatory Breast Cancer: A Population-Based Study
(2018-06-03)
Background. Inflammatory breast cancer (IBC) is a rare but most aggressive breast cancer subtype. The impact of locoregional therapy on survival in IBC is controversial. Methods. Patients with nonmetastatic IBC between ...
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
(2018-11-06)
Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduc- tion and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for ...
Biological significance of RNA-seq and single-cell genomic research in woody plants
(2019-04-01)
RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living organisms. The knowledge gained ...