Browsing Physics by Author "Chen, De"
-
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics
Peng, Lixin; Chen, De; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2009-05-15)Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneously measure levels of Ca-dipicolinic acid (CaDPA) and changes in spore morphology and refractive index during germination ... -
How Moist Heat Kills Spores of Bacillus subtilis
Coleman, William H.; Chen, De; Li, Yong-Qing; Cowan, Ann E.; Setlow, Peter (East Carolina University, 2007-12)Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores ... -
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2007-07)Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many ... -
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
Magge, Anil; Granger, Amanda C.; Wahome, Paul G.; Setlow, Barbara; Vepachedu, Venkata R.; Loshon, Charles A.; Peng, Lixin; Chen, De; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2008-07)Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these ...