Browsing Biochemistry and Molecular Biology by Title
Now showing items 1-20 of 39
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The Actin Binding Protein, Fesselin, is a Member of the Synaptopodin Family
(East Carolina University, 2008-07)Fesselin is a natively unfolded protein that is abundant in avian smooth muscle. Like many natively unfolded proteins, fesselin has multiple binding partners including actin, myosin, calmodulin and α-actinin. Fesselin ... -
The Biochemical Characterization Of Neuronal K+ Channel N-Glycans And Their Role In Regulating K+ Channel Function
(East Carolina University, 2009)The Kv3 and Kv1 subfamilies of voltage–gated K+ channels are critical components which contribute to action potential repolarization throughout the central nervous system. Here, it was shown that both absolutely conserved ... -
Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications
(East Carolina University, 2004-09)Skeletal and cardiac muscle contraction are inhibited by the actin-associated complex of tropomyosin-troponin. Binding of Ca21 to troponin or binding of ATP-free myosin to actin reverses this inhibition. Ca21 and ATP-free ... -
Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.
(East Carolina University, 1999-12)The purpose of this study was to address the paradox of calponin localization with a-actinin and filamin, two proteins with tandem calponin homology (CH) domains, by determining the effect of these proteins on the binding ... -
Characterization of a caldesmon fragment that competes with myosin-ATP binding to actin.
(East Carolina University, 1993-08)The protein caldesmon inhibits actin-activated ATP hydrolysis of myosin and inhibits the binding of myosin*ATP to actin. Afragment isolated from a chymotryptic digest of caldesmon contains features of the intact molecule ... -
Conservation of the regulated structure of folded myosin 2 in species separated by at least 600 million years of independent evolution
(East Carolina University, 2008-04-22)The myosin 2 family of molecular motors includes isoforms regulated in different ways. Vertebrate smooth-muscle myosin is activated by phosphorylation of the regulatory light chain, whereas scallop striated adductor-muscle ... -
The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin
(East Carolina University, 2004-12-07)The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca2+ concentrations. Ca2+ stimulates ATP activity, and at ... -
Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity
(East Carolina University, 1993-01-05)A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. The deoxyguanosine-resistant cells (dGuo-R) were 126-fold less sensitive to deoxyguanosine than the wild-type cells. The IC50 values for araC ... -
The Distribution Of Regulated Actomyosin States Is Central To Cardiac Muscle Regulation And Disturbance Of This Distribution Leads To Congenital Cardiomyopathies
(East Carolina University, 2009)Hypertrophic and restrictive cardiomyopathies are congenital cardiac diseases that have an incidence of over one in five hundred and may lead to sudden cardiac death. One of the main impediments to directed treatment is ... -
Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.
(East Carolina University, 1997-02)3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member ... -
Effects of Exogenous and Autocrine Growth Hormone (hGH) on Prostate Cancer Cell Function
(East Carolina University, 2012)Human growth hormone (hGH) is a major product of the anterior pituitary. In addition to its endocrine activities, hGH overexpression in extra-pituitary tissues has been correlated with the oncogenic behavior of these ... -
Equilibration and Exchange of Fluorescently Labeled Molecules in Skeletal Muscle Fibers Studied Using Confocal Microscopy
(East Carolina University, 1995-04)Diffusion of molecules into skinned muscle fibers is often necessary when studying muscle contraction and its regulation. Usually, it is assumed that diffusion of molecules is fast also in the structured system of a muscle ... -
An examination of the functional role of TMEFF2 in prostate cancer and the translational regulatory mechanisms controlling its expression
(East Carolina University, 2012)Prostate cancer is the most commonly diagnosed cancer in American men and accounts for approximately 11% of cancer-related deaths. Although promising treatment strategies have been developed and are currently being tested ... -
Fesselin and caldesmon: natively unfolded proteins in smooth muscle regulation
(East Carolina University, 2010)Dysregulation of smooth muscle contraction is linked to diseases such as atherosclerosis, asthma, hypertension, urinary incontinence, premature birth and others. The goal of this project is to contribute to our understanding ... -
Fesselin, a Synaptopodin-like Protein, Stimulates Actin Nucleation and Polymerization
(East Carolina University, 2001-11-27)Fesselin is a proline-rich actin binding protein that has recently been isolated from smooth muscle [Leinweber, B. D., Fredricksen, R. S., Hoffman, D. R., and Chalovich, J. M. (1999) J. Muscle Res. Cell Motil. 20, 539–545]. ... -
Fish oil disrupts B cell plasma membrane lateral organization and immunological synapse formation
(East Carolina University, 2012)Fish oil has immunosuppressive properties that could provide treatment for numerous inflammatory and autoimmune disorders. The primary bioactive components of fish oil, which are the n-3 polyunsaturated fatty acids (PUFAs) ... -
A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm
(East Carolina University, 2009-05-15)Fertility and embryonic viability are measures of efficient germ cell growth and development. During oogenesis and spermatogenesis, new proteins are required for both mitotic expansion and differentiation. Qualitative and ... -
HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes
(East Carolina University, 2007-03-30)HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA, HuR is recognized by adapter proteins which then facilitate nuclear export of the ... -
HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype
(East Carolina University, 2009-05-29)In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements in the 3?- untranslated region. HuR may influence expression of its ligand mRNA through regulation of polyadenylation, translocation of the ... -
Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1
(East Carolina University, 2003-06-01)The p21-activated protein kinases (PAKs) have been implicated in cytoskeletal rearrangements and modulation of non-muscle contractility. Little, however, is known about the role of the PAK family members in smooth muscle ...