Browsing Biochemistry and Molecular Biology by Title
Now showing items 36-39 of 39
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Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State
(East Carolina University, 2009-03-18)We examined four cardiomyopathy-causing mutations of troponin I that appear to disturb function by altering the distribution of thin filament states. The R193H (mouse) troponin I mutant had greater than normal actin-activated ... -
Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study
(East Carolina University, 2008-06)Cryoelectron microscopy studies have identified distinct locations of tropomyosin (Tm) within the Ca21-free, Ca21-saturated, and myosin-S1-saturated states of the thin filament. On the other hand, steady-state Förster ... -
Troponin-tropomyosin: an allosteric switch or a steric blocker?
(East Carolina University, 2002-08)The interaction of myosin subfragment 1 (S1) with actin-tropomyosin-troponin (regulated actin) is highly nucleotide dependent. The binding of S1 or S1-ADP (but not S1-ATP nor N,N - -phenylenedimaleimide-modified S1-ATP) ... -
Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes.
(East Carolina University, 1999-03-15)We have demonstrated previously that treatment of 3T3-L1 adipocytes with tumour necrosis factor-alpha (TNF) results in a rapid (4 h) and significant (75–80%) reduction in the rate of transcription of the GLUT4 gene. Control ...