Browsing Dissertations and Theses by Author "Chemistry"
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Synthesis, structure, photophysics, and biological activity of platinum (II) complexes
Vezzu, Dileep Atchyuth Kumar (East Carolina University, 2013)The synthesis, structure, and photophysical properties of luminescent platinum (II) complexes with different coordination patterns, (C^C*N^N), (N^C*N), (N^N*C) and (N^N^C) are reported, where "C^N or N^N" denotes a bidentate ... -
THE DEVELOPMENT OF BETA-ACTIN MUTANTS WITH ALTERED BINDING AFFINITES FOR ATP AND ADP
Kuyateh, Abu-Bakarr (East Carolina University, 2018-12-10)A number of neurodegenerative diseases are accompanied by the formation of cofilin-actin rods in neural cells. These rods can cause synaptic dysfunction and block transport within neurites. The overall goal of this project ... -
THE IMPACT OF GLYCOSYLATION ON ACTIVE SITE STRUCTURE AND ACTIVITY IN THE FUNGUS ENZYME MOLOX
Kostenko, Anastasiia (East Carolina University, 2019-05-01)Lipoxygenases from pathogenic fungi belong to the lipoxygenase family that catalyze the C-H activation of polyunsaturated fatty acids to form diverse hydroperoxides. While the lipoxygenase catalytic domains are structurally ... -
THE SYNTHESIS, STRUCTURE AND PHOTOPHYSICAL PROPERTIES OF PHOSPHORESCENT CYCLOMETALATED N^N^C-COORDINATED PLATINUM COMPLEXES
Mroz, Robert (East Carolina University, 2016-07-25)Phosphorescent materials based on cyclometalated platinum complexes have attracted a great deal of attention because of their potential in chemical, biological and optoelectronic applications, specifically as emitters in ... -
The Thermodynamics of Cd(II) Binding to Human Cardiac Troponin C: Using Mutants to Determine the Location of Cd(II) Binding
Buddo, Katherine A. (East Carolina University, 2017-05-31)Historically, cadmium (Cd) has been used in many different applications from cadmium paint to nickel-cadmium batteries. However, Cd is a nonessential, naturally occurring toxic element due to its low excretion rate and ... -
Theoretical Probe to the Mechanism of Pt-catalyzed C-H Acylation Reaction: Possible Pathways for the Key Acylation Reaction of Platinacycles
Warden, Elizabeth Fae (East Carolina University, 2019-08-23)Transition metal-catalyzed C-H bond functionalization offers a variety of desirable transformations of simple hydrocarbons to more complex compounds with applications being applied in pharmaceuticals to material sciences. ... -
Theoretical studies on the Annexin A1-S100A11 Heterotetramer and Platinum-Mediated C-H Activation Pathways
Sanchez, Wesley (East Carolina University, 2023-07-13)Computational chemistry is a powerful tool for characterizing chemical phenomena when they are difficult to observe in laboratory settings or simply elusive. By employing the fundamental equations of nature, computational ... -
Thermodynamic and spectroscopic studies of Cd²⁺ binding to the regulatory domain and full length human cardiac troponin C (HcTnC) : elucidating plausible Cd²⁺ binding sites
Fulcher, Lindsay Michelle (East Carolina University, 2015)Toxic metals such as cadmium (Cd²⁺) have been shown to bind to and interfere with various calcium (Ca²⁺) binding proteins including the regulatory protein cardiac troponin C (cTnC). Recent structural data has shown that ... -
Thermodynamic Investigation into the Binding Properties of Cardiac Troponin (Human and Bovine)
Haulsee, Zachary Merle (East Carolina University, 2010)Troponin is an integral protein in the mechanism of muscle contraction. In order to induce cardiac muscle contraction, Ca[superscript]2[superscript]+ must bind to the TnC subunit (calcium binding subunit) of troponin to ... -
Thermodynamic Investigation of Copper Binding to the Amyloid-Beta (A[beta]) Peptide
Sacco, Cristina (East Carolina University, 2010)Alzheimer's disease is a fatal and neurodegenerative disease with as many as 5.3 million Americans living with the disease. One of the characteristic hallmarks of Alzheimer's is the amyloid plaques that build up around the ... -
Thermodynamic Investigations into Copper Binding in the N-Terminal Region of the Prion Protein at Low Copper Loadings
Gogineni, Devi Praneetha (East Carolina University, 2010)The prion protein (PrP) is a naturally occurring protein found at high levels in central nervous system (CNS). The misfolding of the PrP is responsible for neurodegenerative diseases called transmissible spongioform ... -
Thermodynamic studies of Ca²⁺ binding to human cardiac troponin C
Skowronsky, Rachel Ann (East Carolina University, 2012)Human Cardiac Troponin C (HcTnC) is an EF hand protein responsible for initiating contraction of the myocardium (heart muscle). The helix-loop-helix motif, characteristic of members of the EF-hand family, allows HcTnC to ... -
Towards Establishing Methods of Synthesis, Purification, and Structural Determination for Human Fibrinogen
Kirby, Nicholas Carter (East Carolina University, 2023-05-04)Fibrinogen is a prevalent blood-based glycoprotein. In its polymerizable form, fibrin, it serves as the structural component of blood clots and helps to incorporate red blood cells, macrophages, and fibroblasts to the ... -
Toxic Metal Interactions with Human Cardiac Troponin C and Cysteine Replacement Mutants: Investigating Potential Cd(II) Binding to Cysteine Thiols Using DTNB Time Course Assays
Fyle, Amanda (East Carolina University, 2021-07-26)Calcium binding proteins contribute to all aspects of cell function by binding to calcium directly. Human Cardiac Troponin C (hcTnC) belongs to the EF-hand family of calcium binding proteins. This specific protein is ... -
Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers
Angermeier, Tori Marena (East Carolina University, 2018-07-24)It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) ... -
Using Fluorescent Probes to Study the Location and Interaction of Disaccharides within Lipid Bilayers
Barker, Morgan Elizabeth (East Carolina University, 2016-04-26)It is well known that certain organisms, such as the tardigrade, are capable of surviving extreme conditions including dehydration, freezing and / or oxygen deficiency. The tardigrades ability to withstand such extreme ... -
Utilizing Isothermal Titration Calorimetry to Quantify the Thermodynamics of Cadmium Mimicry: A Comparison to Calcium
Johnson, Rachel Anne (East Carolina University, 2016-07-20)Isothermal titration calorimetry (ITC) is a powerful calorimetric method which can determine all thermodynamic parameters (K, [Delta]H, [Delta]G, and [Delta]S) for a binding event in a single experiment. More recently, ITC ... -
WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD
McGrath, Jacqueline (East Carolina University, 2010)Intracellular pH plays an important role in many biological processes: receptor-mediated signal transduction, enzymatic activity, cell growth and death, ion transport and homeostasis, calcium regulation, endocytosis, ...