Browsing Physics by Author "Li, Yong-Qing"
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A Clostridium Difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination
Li, Yong-Qing; Li, William (2017-03-08) -
Effects of High-Pressure Treatment on Spores of Clostridium Species
Li, Yong-Qing (2016-09-01) -
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics
Peng, Lixin; Chen, De; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2009-05-15)Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneously measure levels of Ca-dipicolinic acid (CaDPA) and changes in spore morphology and refractive index during germination ... -
Germination, Outgrowth, and Vegetative-Growth Kinetics of Dry-Heat-Treated Individual Spores of Bacillus Species
Li, Yong-Qing (2018-04-01) -
How Moist Heat Kills Spores of Bacillus subtilis
Coleman, William H.; Chen, De; Li, Yong-Qing; Cowan, Ann E.; Setlow, Peter (East Carolina University, 2007-12)Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores ... -
In Vivo Blood Glucose Quantification Using Raman Spectroscopy
Shao, Jingwei; Lin, Manman; Li, Yong-Qing; Li, Xue; Liu, Junxian; Liang, Jianpin; Yao, Huilu (2012)We here propose a novel Raman spectroscopy method that permits the noninvasive measurement of blood glucose concentration. To reduce the effects of the strong background signals produced by surrounding tissue and to obtain ... -
Kinetics of Germination of Individual Spores of as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Zhou, Tingting; Dong, Zhiyang; Setlow, Peter; Li, Yong-Qing (2013)Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of ... -
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2007-07)Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many ... -
Measurement of Mass by Optical Forced Oscillation of Absorbing Particles Trapped in Air
Li, Yong-Qing (2017-05-25) -
Memory of Germinant Stimuli in Bacterial Spores
Wang, Shiwei; Faeder, James R.; Setlow, Peter; Li, Yong-Qing (2015-11)Bacterial spores, despite being metabolically dormant, possess the remarkable capacity to detect nutrients and other molecules in their environment through a biochemical sensory apparatus that can trigger spore germination, ... -
Memory of Germinant Stimuli in Bacterial Spores
Li, Yong-Qing (2015-12-31) -
Optical Pulling, Optical Deflection, and Optical Trapping of Single Airborne Particles using Negative Photophoresis
Hart, Adam (East Carolina University, 2016-05-03)Optical manipulation is the use of light to control the motion of objects. This thesis focuses on the development of optical transportation technology via optical pulling with application to the characterization of single ... -
Raman Characterizations of Red Blood Cells with β-Thalassemia Using Laser Tweezers Raman Spectroscopy
Li, Yong-Qing (2018-09-01) -
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
Magge, Anil; Granger, Amanda C.; Wahome, Paul G.; Setlow, Barbara; Vepachedu, Venkata R.; Loshon, Charles A.; Peng, Lixin; Chen, De; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2008-07)Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these ... -
Single-Cell Analysis Reveals Individual Spore Responses to Simulated Space Vacuum
Li, Yong-Qing (2018-12-01) -
Slow Leakage of Ca-Dipicolinic Acid from Individual Bacillus Spores during Initiation of Spore Germination
Li, Yong-Qing (2015-03-15) -
Stable Optical Trapping and Sensitive Characterization of Nanostructures Using Standing-Wave Raman Tweezers
Li, Yong-Qing (2017-02-17) -
Superdormant Spores of Bacillus Species Have Elevated Wet-Heat Resistance and Temperature Requirements for Heat Activation
Ghosh, Sonali; Zhang, Pengfei; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2009-09)Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, D-glucose, or L-valine, respectively, germinate ...