Browsing Biochemistry and Molecular Biology by Title
Now showing items 5-24 of 82
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Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications
(East Carolina University, 2004-09)Skeletal and cardiac muscle contraction are inhibited by the actin-associated complex of tropomyosin-troponin. Binding of Ca21 to troponin or binding of ATP-free myosin to actin reverses this inhibition. Ca21 and ATP-free ... -
Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.
(East Carolina University, 1999-12)The purpose of this study was to address the paradox of calponin localization with a-actinin and filamin, two proteins with tandem calponin homology (CH) domains, by determining the effect of these proteins on the binding ... -
Catabolite repression control (crc) gene and pseudomonas virulence
(2002-08-22)A method of screening for compounds that inhibit the virulence of Pseudomonas bacteria comprises the steps of: providing a culture medium comprising Pseudomonas bacteria; administering a test compound to said bacteria; and ... -
Characterization of a caldesmon fragment that competes with myosin-ATP binding to actin.
(East Carolina University, 1993-08)The protein caldesmon inhibits actin-activated ATP hydrolysis of myosin and inhibits the binding of myosin*ATP to actin. Afragment isolated from a chymotryptic digest of caldesmon contains features of the intact molecule ... -
Composition and formulations and their use as nociceptic, anti-anxiolytic and anabolic agents
(2000-07-13)Composition and formulations comprising a first agent such as folinic acid, pharmaceutically acceptable salts thereof or mixtures thereof, and a second agent(s) such as analgesics, muscle relaxants, mood disorder agents, ... -
Conservation of the regulated structure of folded myosin 2 in species separated by at least 600 million years of independent evolution
(East Carolina University, 2008-04-22)The myosin 2 family of molecular motors includes isoforms regulated in different ways. Vertebrate smooth-muscle myosin is activated by phosphorylation of the regulatory light chain, whereas scallop striated adductor-muscle ... -
The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin
(East Carolina University, 2004-12-07)The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca2+ concentrations. Ca2+ stimulates ATP activity, and at ... -
Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity
(East Carolina University, 1993-01-05)A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. The deoxyguanosine-resistant cells (dGuo-R) were 126-fold less sensitive to deoxyguanosine than the wild-type cells. The IC50 values for araC ... -
DISCRIMINATING BETWEEN CARDIOLIPIN CONCENTRATION AND ACYL CHAIN COMPOSITION ON MEMBRANE BIOPHYSICAL ORGANIZATION
(East Carolina University, 2018-11-28)The pathogenesis of cardiovascular diseases (CVDs) is driven, in part, from impairment in myocardial energy metabolism. There is convincing evidence that myocardial metabolic abnormalities are fundamentally driven by ... -
Disruption of pqs synthesis using precursor analogs
(2004-01-08)The present invention concerns methods of detecting bacterial infections as well as methods of treating such infections with compounds such as methyl anthranilate. The detection and treatment of Pseudomonas is particularly ... -
The Distribution Of Regulated Actomyosin States Is Central To Cardiac Muscle Regulation And Disturbance Of This Distribution Leads To Congenital Cardiomyopathies
(East Carolina University, 2009)Hypertrophic and restrictive cardiomyopathies are congenital cardiac diseases that have an incidence of over one in five hundred and may lead to sudden cardiac death. One of the main impediments to directed treatment is ... -
Docosahexaenoic acid-derived metabolites target the B-cell driven immune response in obese mice
(East Carolina University, 2019-06-07)Obesity is associated with an impaired humoral immune response. As B cells mediate the humoral immune response, they are major cellular targets in diet induced obesity models. Several studies have established that B cell ... -
Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.
(East Carolina University, 1997-02)3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member ... -
Effects of Exogenous and Autocrine Growth Hormone (hGH) on Prostate Cancer Cell Function
(East Carolina University, 2012)Human growth hormone (hGH) is a major product of the anterior pituitary. In addition to its endocrine activities, hGH overexpression in extra-pituitary tissues has been correlated with the oncogenic behavior of these ... -
Equilibration and Exchange of Fluorescently Labeled Molecules in Skeletal Muscle Fibers Studied Using Confocal Microscopy
(East Carolina University, 1995-04)Diffusion of molecules into skinned muscle fibers is often necessary when studying muscle contraction and its regulation. Usually, it is assumed that diffusion of molecules is fast also in the structured system of a muscle ...