Browsing Biochemistry and Molecular Biology by Title
Now showing items 43-62 of 82
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Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites
(East Carolina University, 2016-07-13)Catalysis occurring in a multifunctional enzyme at spatially distinct active sites is controlled by an array of factors, including the structure of the enzyme, ligand binding, and productive interaction of substrates to ... -
Kinetics of Binding of Caldesmon to Actin
(East Carolina University, 1995-04-28)The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ... -
Lipid mediators of insulin resistance: ceramide signalling down-regulates GLUT4 gene transcription in 3T3-L1 adipocytes.
(East Carolina University, 1996-10-01)We have previously demonstrated that chronic exposure of 3T3- L1 adipocytes to tumour necrosis factor-α (TNF) resulted in a marked decrease (~90%) in cellular GLUT4 (insulin-responsive glucose transporter) mRNA content as ... -
Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity
(East Carolina University, 1992-08-15)Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several ... -
A Long Helix from the Central Region of Smooth Muscle Caldesmon
(East Carolina University, 1991-07-25)The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic properties and the electron ... -
Low adenosine anti-sense oligonucleotide agent, composition, kit and treatments
(2000-02-24)A composition comprises a nucleic acid comprising an oligo anti-sense to a target such as polypeptide(s) associated with an ailment afflicting lung airways, genes and mRNAs encoding them, genomic and mRNA flanking regions, ... -
Measles vaccine vectors and methods of use
(2003-03-20)The present invention provides a novel vaccine expressing a fusion of the measles hemagglutinin (H) protein and the complement component, C3d, to enhance the titers of neutralizing antibody. Plasmids were generated that ... -
Method for the synthesis of 3-substituted indolizine and benzoindolizine compounds
(2006-07-13)A method of making a compound of Formula (I) comprises reacting a compound of Formula (II) with a compound such as R1OH or R1SH, to produce said compound of Formula (I). Compounds of Formula (I) are useful, among other ... -
Molecular mechanisms of TMEFF2 action in Prostate Cancer
(East Carolina University, 2013)The transmembrane protein with epidermal growth factor and two follistatin motifs 2 (TMEFF2) is an evolutionarily conserved type I transmembrane protein expressed in the embryo and limited adult tissues, mainly the brain ... -
Multiple Translation Factor eIF4G (IFG-1) Isoforms are Required for the Apoptosome-Dependent Activation of Germ Cell Apoptosis
(East Carolina University, 2010)Apoptosis is a naturally occurring process during animal development required for the programmed killing and removal of injured cells. Cellular insult induces a switch in translation that allows for the rapid synthesis ... -
N-3 polyunsaturated fatty acids differentially enhance B-cell mediated immunity in lean and obese mice
(East Carolina University, 2014)Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are bioactive n-3 polyunsaturated fatty acids (PUFAs) in fish oil that exert immunomodulatory effects. The general paradigm suggests n-3 PUFAs exert immunosuppressive ... -
Negative Charges at Protein Kinase C Sites of Troponin I Stabilize the Inactive State of Actin
(East Carolina University, 2008-01-15)Alterations in the troponin complex can lead to increases or decreases in contractile activity. Most mutations of troponin that cause hypertrophic cardiomyopathy increase the activity of cardiac muscle fibers. In at least ... -
Neurotransmitter Metabolism by Monoamine Oxidase in Porcine Heart Differs by Location and is Increased with Obesity/Metabolic Syndrome
(East Carolina University, 2017-05-05)Monoamine Oxidase (MAO), an outer mitochondrial enzyme, catalyzes the oxidative deamination of catecholamines and other biological amines, and in the process, produces a reactive aldehyde and H2O2. Our lab has recently ... -
NMR method for monitoring changes in the core of lipoprotein particles in metabolism and disease
(2017-01-14)A method is disclosed for measuring the properties of protein and lipoprotein elements in a sample. The method includes the of placing a small volume of a sample into a NMR instrument tuned to measure a particular nucleus, ... -
Novel autoinducer molecules and uses therefor
(2002-03-07)Novel bacterial quinolone signal molecules and, more particularly, pseudomonas quinolone signal ('PQS') molecules, e.g., 2-heptyl-3hydroxy-4-quinolone, and analogs and derivatives thereof are described, Therapeutic ... -
Novel diagnostic and analytical applications of benchtop time-domain NMR.
(East Carolina University, 2015)High-resolution nuclear magnetic resonance (NMR) spectroscopy is a powerful technique, yet practical application outside of the research laboratory has been limited due to the high cost and complexity of the instrumentation. ... -
Post-Transcriptional Regulation of mRNA Metabolism during Differentiation of 3T3-L1 Cells: Role of HuR
(East Carolina University, 2010)Our studies address early control points in the differentiation process that are necessary for expression as well as the maintenance of the adipocyte phenotype. We have focused on the post-transcriptional regulation of ...