Browsing Biochemistry and Molecular Biology by Author "Pekala, Phillip H."
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Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.
Jain, Renu G.; Andrews, Lucy G.; McGowan, Kevin M.; Pekala, Phillip H.; Keene, Jack D. (East Carolina University, 1997-02)3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member ... -
HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes
Jones, Heath; Carver, Melinda; Pekala, Phillip H. (East Carolina University, 2007-03-30)HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA, HuR is recognized by adapter proteins which then facilitate nuclear export of the ... -
HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype
Karschner, Vesna Ann; Pekala, Phillip H. (East Carolina University, 2009-05-29)In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements in the 3?- untranslated region. HuR may influence expression of its ligand mRNA through regulation of polyadenylation, translocation of the ... -
Insulin responsiveness in skeletal muscle is determined by glucose transporter (Glut4) protein level.
Kern, Marialice; Wells, James A.; Stephens, Jacqueline M.; Elton, Charles W.; Friedman, Jacob E.; Tapscott, Edward B.; Pekala, Phillip H.; Dohm, G. Lynis (East Carolina University, 1990-09-01)Glucose transport in skeletal muscle is mediated by two distinct transporter isoforms, designated muscle/adipose glucose transporter (Glut4) and erythrocyte/HepG2/brain glucose transporter (Glutl), which differ in both ... -
Lipid mediators of insulin resistance: ceramide signalling down-regulates GLUT4 gene transcription in 3T3-L1 adipocytes.
Long, Sheree D.; Pekala, Phillip H. (East Carolina University, 1996-10-01)We have previously demonstrated that chronic exposure of 3T3- L1 adipocytes to tumour necrosis factor-α (TNF) resulted in a marked decrease (~90%) in cellular GLUT4 (insulin-responsive glucose transporter) mRNA content as ... -
Post-Transcriptional Regulation of mRNA Metabolism during Differentiation of 3T3-L1 Cells: Role of HuR
Karschner, Vesna Ann (East Carolina University, 2010)Our studies address early control points in the differentiation process that are necessary for expression as well as the maintenance of the adipocyte phenotype. We have focused on the post-transcriptional regulation of ... -
Regulation of lipoprotein lipase mRNA content in 3T3-L1 cells by tumour necrosis factor.
Cornelius, Peter; Enerback, Sven; Bjursell, Gunnar; Olivecrona, Thomas; Pekala, Phillip H. (East Carolina University, 1988-02-01)Tumour necrosis factor (TNF) was previously shown to suppress lipoprotein lipase (LPL) synthesis and activity in 3T3-L1 adipocytes. The present study examined the effect ofTNF on amounts ofmRNA for LPL in 3T3-Ll cells. ... -
Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes.
Jain, Renu G.; Police, Shailaja; Phelps, Kelle; Pekala, Phillip H. (East Carolina University, 1999-03-15)We have demonstrated previously that treatment of 3T3-L1 adipocytes with tumour necrosis factor-alpha (TNF) results in a rapid (4 h) and significant (75–80%) reduction in the rate of transcription of the GLUT4 gene. Control ...