Browsing Microbiology and Immunology by Title
Now showing items 11-30 of 100
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The Bacteroides mobilizable transposon Tn4555 integrates by a site-specific recombination mechanism similar to that of the gram-positive bacterial element Tn916
(East Carolina University, 1997-04)The Bacteroides mobilizable transposon Tn4555 is a 12.2-kb molecule that encodes resistance to cefoxitin. Conjugal transposition is hypothesized to occur via a circular intermediate and is stimulated by coresident tetracycline ... -
Base-By-Base: Single nucleotide-level analysis of whole viral genome alignments
(East Carolina University, 2004-07-14)Background: With ever increasing numbers of closely related virus genomes being sequenced, it has become desirable to be able to compare two genomes at a level more detailed than gene content because two strains of an ... -
The bhuTUV and bhuO genes play vital roles in the ability of Brucella abortus to use heme as an iron source and are regulated in an iron-responsive manner by RirA and Irr
(East Carolina University, 2012)Brucella abortus is a Gram negative intracellular pathogen that causes the zoonotic disease brucellosis. Antibiotic treatment for brucellosis in humans is prolonged and sometimes followed by relapses. Currently, the United ... -
Biochemical and genetic analyses of a catalase from the anaerobic bacterium Bacteroides fragilis.
(East Carolina University, 1995-06)A single catalase enzyme was produced by the anaerobic bacterium Bacteroides fragilis when cultures at late log phase were shifted to aerobic conditions. In anaerobic conditions, catalase activity was detected ... -
Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants.
(East Carolina University, 1995-01)We report the isolation and preliminary phenotypic characterization of manganese-resistant Bordetella bronchiseptica mutants with respect to deregulation of siderophore and iron-regulated protein expression. The fur gene ... -
Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing
(East Carolina University, 2008-08)Experiments requiring strong repression and precise control of cloned genes can be difficult to conduct because of the relatively high basal level of expression of currently employed promoters. We report the construction ... -
The Brucella abortus Cu,Zn Superoxide Dismutase Is Required for Optimal Resistance to Oxidative Killing by Murine Macrophages and Wild-Type Virulence in Experimentally Infected Mice
(East Carolina University, 2005-05)Two-dimensional gel electrophoretic analysis of cell lysates from Brucella abortus 2308 and the isogenic hfq mutant Hfq3 revealed that the RNA binding protein Hfq (also known as host factor I or HF-I) is required for the ... -
Butyl Rubber Stoppers Increase the Shelf Life of Prereduced, Anaerobically Sterilized Media
(East Carolina University, 1977-05)Butyl rubber stoppers as compared with neoprene or black rubber stoppers significantly increased the shelf life of prereduced, anaerobically sterilized media for growth of obligately anaerobic bacteria. Originally published ... -
Cecal and fecal bacterial flora of the Mongolian gerbil and the chinchilla.
(East Carolina University, 1988-05)The Mongolian gerbil is being increasingly used as a laboratory animal and as a pet. Both chinchillas and gerbils are used as animal models for otitis media and other otic research. Previously, only incomplete information ... -
Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis
(East Carolina University, 1998-11)A Bacteroides fragilis mutant resistant to hydrogen peroxide and alkyl peroxide was isolated by enrichment in increasing concentrations of hydrogen peroxide. The mutant strain was constitutively resistant to 100 mM H2O2 ... -
Characterization of Bacteroides fragilis Hemolysins and Regulation and Synergistic Interactions of HlyA and HlyB
(East Carolina University, 2006-04)This study describes the presence of 10 hemolysin orthologs in the genome of the opportunistic human anaerobic pathogen Bacteroides fragilis, which is currently classified as a nonhemolytic bacterium. The hemolysins were ... -
Characterization of the avian GLUT1 glucose transporter: differential regulation of GLUT1 and GLUT3 in chicken embryo fibroblasts.
(East Carolina University, 1995-11)Vertebrate cells that are transformed by oncogenes such as v-src or are stimulated by mitogens have increased rates of glucose uptake. In rodent cels, the mechanisms whereby glucose transport is up-regulated are well ... -
Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen
(East Carolina University, 2006-07)The opportunistic pathogen Bacteroides fragilis is a commensal organism in the large intestine, where it utilizes both dietary and host-derived polysaccharides as a source of carbon and energy. In this study, a four-gene ... -
Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens.
(East Carolina University, 1995-07)The gene encoding a conjugated bile acid hydrolase (CBAH) from Clostridium perfringens 13 has been cloned and expressed in Escherichia coli, and its nucleotide sequence has been determined. Nucleotide and predicted amino ... -
Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.
(East Carolina University, 1993-11)Bacteroides frgiglis CS30 is a clinical isolate resistant to high concentrations of benzylpenicillin and cephaloridine but not to cephamycin or penem antibiotics. beta-Lactam resistance is mediated by a chromosomally encoded ... -
Cloning and Characterization of the Pseudomonas aeruginosa zwf Gene Encoding Glucose-6-Phosphate Dehydrogenase, an Enzyme Important in Resistance to Methyl Viologen (Paraquat)
(East Carolina University, 1998-04)In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD1- or NADP1-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. ... -
Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa
(East Carolina University, 2000-02)Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in ... -
Deficiency of a Sinorhizobium meliloti bacA Mutant in Alfalfa Symbiosis Correlates with Alteration of the Cell Envelope
(East Carolina University, 2002-10)The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA ... -
Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins
(East Carolina University, 2008-07)Kaposi’s sarcoma-associated herpesvirus (KSHV) has two modes replication: latent and lytic replication. Reactivation from latency is dictated, in part, by the cell cycle. Herein, we have attempted to delineate the importance ... -
Effect of in vitro transcription on cruciform stability.
(East Carolina University, 1990-05-11)We have investigated the effect of in vitro transcription on cruciform stability. Replicative form DNA of 4X174 strain ins6240, containing a 48 bp synthetic palindrome in the J-F intercistronic region, was supercoiled in ...