Browsing Microbiology and Immunology by Title
Now showing items 85-104 of 114
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Raf-Induced Vascular Endothelial Growth Factor Augments Kaposi's Sarcoma-Associated Herpesvirus Infection
(East Carolina University, 2004-12)Recombinant green fluorescent protein encoding Kaposi’s sarcoma-associated herpesvirus (rKSHV.152) infection of -estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ B-Raf[FF]:ER (expressing a weaker form of ... -
Raman spectroscopy: the gateway into tomorrow's virology
(East Carolina University, 2006-06-28)In the molecular world, researchers act as detectives working hard to unravel the mysteries surrounding cells. One of the researchers' greatest tools in this endeavor has been Raman spectroscopy. Raman spectroscopy is a ... -
Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation
(East Carolina University, 2009-08)Kaposi's sarcoma-associated herpesvirus (KSHV) has both latent and lytic phases of replication. The molecular switch that triggers a reactivation is still unclear. Cells from S phase of cell cycle provide apt conditions ... -
RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst
(East Carolina University, 2006-07)Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in ... -
The Redox-Sensitive Transcriptional Activator OxyR Regulates the Peroxide Response Regulon in the Obligate Anaerobe Bacteroides fragilis
(East Carolina University, 2000-09)The peroxide response-inducible genes ahpCF, dps, and katB in the obligate anaerobe Bacteroides fragilis are controlled by the redox-sensitive transcriptional activator OxyR. This is the first functional oxidative ... -
Regulation of KSHV replication by glycoprotein B: chronicles of a virus and its host
(East Carolina University, 2011)Kaposi's sarcoma-associated herpesvirus (KSHV), the causative agent of three cancers: Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and the plasmablastic form of multicentric Castleman disease (MCD), shares many ... -
The regulation of anthranilate synthesis for the production of pqs in pseudomonas aeruginosa
(East Carolina University, 2013)The ubiquitous bacterium P. aeruginosa is an important human pathogen that causes devastating infections in immunocompromised patients and chronic infections in cystic fibrosis patients. The ability of P. aeruginosa to ... -
Regulation of Bacteriodes fragilis katB mRNA by oxidative stress and carbon limitation.
(East Carolina University, 1997-11)Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The ... -
Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa
(East Carolina University, 2005-07)Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in cystic fibrosis patients and is a major source of nosocomial infections. This bacterium controls many virulence factors by using ... -
Review: Carbon Nanotubes and Chronic Granulomatous Disease
(2014-06-23)Use of nanomaterials in manufactured consumer products is a rapidly expanding industry and potential toxicities are just beginning to be explored. Combustion-generated multiwall carbon nanotubes (MWCNT) or nanoparticles ... -
Role of the Iron Storage Ferritins Dps and DpsL during the Prolonged Oxidative Stress Response of Bacteroides fragilis
(East Carolina University, 2015-12-02)Bacteroides fragilis is a Gram negative anaerobe and member of the human intestinal tract microbiome. B. fragilis serves many beneficial roles within the intestinal tract; however, its translocation to the peritoneal cavity ... -
Roles of the RAF/MEK/ERK Pathway in Cell Growth, Malignant Transformation and Drug Resistance
(East Carolina University, 2007-08)Growth factors and mitogens use the Ras/Raf/MEK/ERK signaling cascade to transmit signals from their receptors to regulate gene expression and prevent apoptosis. Some components of these pathways are mutated or aberrantly ... -
Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids
(East Carolina University, 2004-04-06)Sinorhizobium meliloti, a legume symbiont, and Brucella abortus, a phylogenetically related mammalian pathogen, both require the bacterial-encoded BacA protein to establish chronic intracellular infections in their respective ... -
Solubility and Bioactivity of the Pseudomonas Quinolone Signal Are Increased by a Pseudomonas aeruginosa-Produced Surfactant
(East Carolina University, 2005-02)Pseudomonas aeruginosa is a gram-negative bacterium that causes serious infections in immunocompromised individuals and cystic fibrosis patients. This opportunistic pathogen controls many of its virulence factors and ... -
Subcellular fractionation method to study endosomal trafficking of Kaposi’s sarcoma-associated herpesvirus
(2016-01-15)Background: Virus entry involves multiple steps and is a highly orchestrated process on which successful infection collectively depends. Entry processes are commonly analyzed by monitoring internalized virus particles ... -
Synergistic Proapoptotic Activity of Recombinant Trail Plus the AKT Inhibitor Perifosine in Acute Myelogenous Leukemia Cells
(East Carolina University, 2008-11-15)To potentiate the response of acute myelogenous leukemia (AML) cells to TNF-Related Apoptosis- Inducing Ligand (TRAIL) cytotoxicity, we have examined the efficacy of a combination with perifosine, a novel phosphatidylinositol ... -
Targeting prostate cancer based on signal transduction and cell cycle pathways
(East Carolina University, 2008-06-15)Prostate cancer remains a leading cause of death in men despite increased capacity to diagnose at earlier stages. After prostate cancer has become hormone independent, which often occurs after hormonal ablation therapies, ... -
Targeting the leukemic stem cell: the Holy Grail of leukemia therapy
(East Carolina University, 2009-01)Since the discovery of leukemic stem cells (LSCs) over a decade ago, many of their critical biological properties have been elucidated, including their distinct replicative properties, cell surface phenotypes, their ... -
Targeting the RAF/MEK/ERK, PI3K/AKT and P53 pathways in hematopoietic drug resistance
(East Carolina University, 2007) -
Thioredoxin Reductase Is Essential for Thiol/Disulfide Redox Control and Oxidative Stress Survival of the Anaerobe Bacteroides fragilis
(East Carolina University, 2007-11)Results of this study showed that the anaerobic, opportunistic pathogen Bacteroides fragilis lacks the glutathione/glutaredoxin redox system and possesses an extensive number of putative thioredoxin (Trx) orthologs. ...