Browsing Microbiology and Immunology by Title
Now showing items 21-40 of 100
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Characterization of Bacteroides fragilis Hemolysins and Regulation and Synergistic Interactions of HlyA and HlyB
(East Carolina University, 2006-04)This study describes the presence of 10 hemolysin orthologs in the genome of the opportunistic human anaerobic pathogen Bacteroides fragilis, which is currently classified as a nonhemolytic bacterium. The hemolysins were ... -
Characterization of the avian GLUT1 glucose transporter: differential regulation of GLUT1 and GLUT3 in chicken embryo fibroblasts.
(East Carolina University, 1995-11)Vertebrate cells that are transformed by oncogenes such as v-src or are stimulated by mitogens have increased rates of glucose uptake. In rodent cels, the mechanisms whereby glucose transport is up-regulated are well ... -
Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen
(East Carolina University, 2006-07)The opportunistic pathogen Bacteroides fragilis is a commensal organism in the large intestine, where it utilizes both dietary and host-derived polysaccharides as a source of carbon and energy. In this study, a four-gene ... -
Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens.
(East Carolina University, 1995-07)The gene encoding a conjugated bile acid hydrolase (CBAH) from Clostridium perfringens 13 has been cloned and expressed in Escherichia coli, and its nucleotide sequence has been determined. Nucleotide and predicted amino ... -
Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.
(East Carolina University, 1993-11)Bacteroides frgiglis CS30 is a clinical isolate resistant to high concentrations of benzylpenicillin and cephaloridine but not to cephamycin or penem antibiotics. beta-Lactam resistance is mediated by a chromosomally encoded ... -
Cloning and Characterization of the Pseudomonas aeruginosa zwf Gene Encoding Glucose-6-Phosphate Dehydrogenase, an Enzyme Important in Resistance to Methyl Viologen (Paraquat)
(East Carolina University, 1998-04)In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD1- or NADP1-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. ... -
Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa
(East Carolina University, 2000-02)Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in ... -
Deficiency of a Sinorhizobium meliloti bacA Mutant in Alfalfa Symbiosis Correlates with Alteration of the Cell Envelope
(East Carolina University, 2002-10)The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA ... -
Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins
(East Carolina University, 2008-07)Kaposi’s sarcoma-associated herpesvirus (KSHV) has two modes replication: latent and lytic replication. Reactivation from latency is dictated, in part, by the cell cycle. Herein, we have attempted to delineate the importance ... -
Effect of in vitro transcription on cruciform stability.
(East Carolina University, 1990-05-11)We have investigated the effect of in vitro transcription on cruciform stability. Replicative form DNA of 4X174 strain ins6240, containing a 48 bp synthetic palindrome in the J-F intercistronic region, was supercoiled in ... -
Effects of palindromes on in vivo DNA replication and mutagenesis in bacteriophage phi X174 RF DNA.
(East Carolina University, 1988-11-25)Bacteriophage IX174 mutants with insertions of palindromic DNA sequences are rapidly outgrown by competing wild-type phage (Muller & Turnage, J. Mol. Biol. 189: 285). The basis for this defect was investigated and found ... -
Enforced expression of HoxA9 in b-lineage all promotes survival and proliferation of leukemic cells
(East Carolina University, 2009)HoxA9 is frequently overexpressed in acute myeloid leukemia, myelodysplastic syndrome and a subset of acute lymphoblastic leukemia. In mouse models, HoxA9 has been shown to promote leukemogenesis. In spite of a seemingly ... -
Enhanced Avidity Maturation of Antibody to Human Immunodeficiency Virus Envelope: DNA Vaccination with gp120-C3d Fusion Proteins
(East Carolina University, 2001-06-10)DNA vaccination can elicit both humoral and cellular immune responses and can confer protection against several pathogens. However, DNA vaccines expressing the envelope (Env) protein of human immunodeficiency virus (HIV) ... -
Enhancement of Antibodies to the Human Immunodeficiency Virus Type 1 Envelope by Using the Molecular Adjuvant C3d
(East Carolina University, 2003-02)DNA vaccines expressing the envelope (Env) protein of the human immunodeficiency virus have been relatively ineffective at generating high-titer, long-lasting, neutralizing antibodies in a variety of animal models. In this ... -
Essential Role of the Iron-Regulated Outer Membrane Receptor FauA in Alcaligin Siderophore-Mediated Iron Uptake in Bordetella Species
(East Carolina University, 1999-10)Phenotypic analysis using heterologous host systems localized putative Bordetella pertussis ferric alcaligin transport genes and Fur-binding sequences to a 3.8-kb genetic region downstream from the alcR regulator gene. ... -
The Flat-Ribbon Configuration of the Periplasmic Flagella of Borrelia burgdorferi and Its Relationship to Motility and Morphology
(East Carolina University, 2009-01)Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical ... -
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa
(East Carolina University, 2002-12)A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in ... -
Generation and characterization of the humoral immune response to DNA immunization with a chimeric beta-amyloid-interleukin-4 minigene
(East Carolina University, 2003-12)Active immunization with fibrillar beta-amyloid peptide (AB42) as well as passive transfer of anti-AB antibodies significantly reduces AB plaque deposition, neuritic dystrophy, and astrogliosis in the brain of mutant amyloid ... -
Genetic and biochemical analysis of a novel Ambler class A β-Lactamase responsible for cefoxitin resistance in Bacteroides species.
(East Carolina University, 1993-05)A clinical isolate of Bacteroides vulgatus was resistant to tetracycline, clindamycin, ampicillin, cephaloridine, cefoxitin, and other 13-lactam antibiotics except imipenem. 13-Lactam resistance was mediated by a ... -
The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa
(East Carolina University, 2000-01)The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite ...