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Now showing items 61-70 of 268
Autolysis and Autoaggregation in Pseudomonas aeruginosa Colony Morphology Mutants
(East Carolina University, 2002-12)
Two distinctive colony morphologies were noted in a collection of Pseudomonas aeruginosa transposon insertion mutants. One set of mutants formed wrinkled colonies of autoaggregating cells. Suppressor analysis of a subset ...
Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing
(East Carolina University, 2008-08)
Experiments requiring strong repression and precise control of cloned genes can be difficult to conduct because of the relatively high basal level of expression of currently employed promoters. We report the construction ...
Deficiency of a Sinorhizobium meliloti bacA Mutant in Alfalfa Symbiosis Correlates with Alteration of the Cell Envelope
(East Carolina University, 2002-10)
The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA ...
Proapoptotic Activity and Chemosensitizing Effect of the Novel Akt Inhibitor (2S)-1-(1H-Indol-3-yl)-3-[5-(3-methyl-2H-indazol-5-yl)pyridin-3-yl]oxypropan2-amine (A443654) in T-Cell Acute Lymphoblastic Leukemia
(East Carolina University, 2008-09)
Constitutively activated AKT kinase is a common feature of T-cell acute lymphoblastic leukemia (T-ALL). Here, we report that the novel AKT inhibitor (2S)-1-(1H-indol-3-yl)-3-[5-(3-methyl-2Hindazol-5-yl)pyridin-3-yl]oxypr ...
Opsonized Virulent Brucella abortus Replicates within Nonacidic, Endoplasmic Reticulum-Negative, LAMP-1-Positive Phagosomes in Human Monocytes
(East Carolina University, 2005-06)
Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucellaspecific ...
Two Distinct Pathways Supply Anthranilate as a Precursor of the Pseudomonas Quinolone Signal
(East Carolina University, 2007-05)
Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections in immunocompromised patients and those with cystic fibrosis (CF). This gram-negative bacterium uses multiple cell-to-cell signals to control ...
Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa
(East Carolina University, 1999-09-28)
Numerous species of bacteria use an elegant
regulatory mechanism known as quorum sensing to control
the expression of specific genes in a cell-density dependent
manner. In Gram-negative bacteria, quorum sensing ...
Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis
(East Carolina University, 1998-11)
A Bacteroides fragilis mutant resistant to hydrogen peroxide and alkyl peroxide was isolated by enrichment
in increasing concentrations of hydrogen peroxide. The mutant strain was constitutively resistant to 100 mM
H2O2 ...
A Multicomponent System Is Required for Tetracycline-Induced Excision of Tn4555
(East Carolina University, 2004-01)
Bacteroides spp. are the predominant organisms in the intestinal tract, and they also are important opportunistic
pathogens. Antibiotic therapy of Bacteroides infections often is complicated by the prevalence of
drug-resistant ...
Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.
(East Carolina University, 1994-08)
The gene, cbl, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from Bacteroides uniforinis WAL-7088. The nucleotide sequence was determined, and the cbl structural gene was ...