Browsing Brody School of Medicine by Issue Date
Now showing items 67-86 of 1484
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Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens.
(East Carolina University, 1995-07)The gene encoding a conjugated bile acid hydrolase (CBAH) from Clostridium perfringens 13 has been cloned and expressed in Escherichia coli, and its nucleotide sequence has been determined. Nucleotide and predicted amino ... -
Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus.
(East Carolina University, 1995-09)OBJECTIVE: This report documents that the gastric bypass operation provides long-term control for obesity and diabetes. SUMMARY BACKGROUND DATA: Obesity and diabetes, both notoriously resistant to medical therapy, continue ... -
Postvaccinial (influenza) disseminated encephalopathy (Brown-Sequard syndrome).
(East Carolina University, 1995-09)This article reports a case of Brown-Sequard syndrome that occurred in a patient following the administration of trivalent influenza vaccine. The patient responded well to intravenous steroids and physical therapy. This ... -
Phorbol esters induce death in MCF-7 breast cancer cells with altered expression of protein kinase C isoforms. Role for p53-independent induction of gadd-45 in initiating death.
(East Carolina University, 1995-10)Protein kinase C (PKC) modulates growth, differentiation, and apoptosis in a cell-specific fashion. Overexpression of PKC-a in MCF-7 breast cancer cells (MCF-7-PKC-a cell) leads to expression of a more transformed phenotype. ... -
Characterization of the avian GLUT1 glucose transporter: differential regulation of GLUT1 and GLUT3 in chicken embryo fibroblasts.
(East Carolina University, 1995-11)Vertebrate cells that are transformed by oncogenes such as v-src or are stimulated by mitogens have increased rates of glucose uptake. In rodent cels, the mechanisms whereby glucose transport is up-regulated are well ... -
The ornithine decarboxylase gene odc is required for alcaligin siderophore biosynthesis in Bordetella spp.: putrescine is a precursor of alcaligin.
(East Carolina University, 1996-01)Chromosomal insertions defining Bordetella bronchiseptica siderophore phenotypic complementation group III mutants BRM3 and BRM5 were found to reside approximately 200 to 300 bp apart by restriction mapping of cloned genomic ... -
Familial cryptic translocation resulting in Angelman syndrome:implications for imprinting or location of the Angelman gene?
(East Carolina University, 1996-04)Angelman syndrome (AS) is associated with a loss of maternal genetic information, which typically occurs as a result of a deletion at 15ql1-q13 or paternal uniparental disomy of chromosome 15. We report a patient with AS ... -
Attenuated viruses and method of making the same
(1996-04-18)Disclosed are attenuated viruses, not naturally occurring, that contain one or more additional methylation sites in the genome of the virus compared to the corresponding wild-type virus. Preferably, the methylation sites ... -
Method of inhibiting carcinogenesis by treatment with dehydroepiandrosterone and analogs thereof
(1996-06-18)A method of combatting cancer in a subject comprising administering to said subject dehydroepiandrosterone (DHEA) or an analog thereof in an amount effective to combat cancer is disclosed in which heart failure induced by ... -
Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.
(East Carolina University, 1996-08)Bordetella bronchiseptica mutants BRM1, BRM6, and BRM9 fail to produce the native dihydroxamate siderophore alcaligin. A 4.5-kb BamHI-SmaI Bordetella pertussis genomic DNA fragment carried multiple genes required to restore ... -
Method of treating asthma
(1996-09-10)A method of treating asthma in a subject in need of such treatment comprises contacting Asthma Reversal Factor to the respiratory epithelium of the subject. The active agent is preferably contacted to the subject's respiratory ... -
The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.
(East Carolina University, 1996-10)The gene (crc) responsible for catabolite repression control in Pseudomonas aeruginosa has been cloned and sequenced. Flanking the crc gene are genes encoding orotate phosphoribosyl transferase (pyrE) and RNase PH (rph). ... -
A two-component response regulator, gltR, is required for glucose transport activity in Pseudomonas aeruginosa PAO1.
(East Carolina University, 1996-10)A 729-bp open reading frame (gltR) was identified in Pseudomonas aeruginosa PAO1 that encodes a product homologous to the two-component response regulator family of proteins. Disruption of gltR caused loss of glucose ... -
Lipid mediators of insulin resistance: ceramide signalling down-regulates GLUT4 gene transcription in 3T3-L1 adipocytes.
(East Carolina University, 1996-10-01)We have previously demonstrated that chronic exposure of 3T3- L1 adipocytes to tumour necrosis factor-α (TNF) resulted in a marked decrease (~90%) in cellular GLUT4 (insulin-responsive glucose transporter) mRNA content as ... -
Oxidative stress response in an anaerobe, Bacteroides fragilis: a role for catalase in protection against hydrogen peroxide.
(East Carolina University, 1996-12)Survival of Bacteroides fragilis in the presence of oxygen was dependent on the ability of bacteria to synthesize new proteins, as determined by the inhibition of protein synthesis after oxygen exposure. The B. fragilis ... -
Comparison of identifications of human and animal source gram-negative bacteria by API 20E and crystal E/NF systems.
(East Carolina University, 1997-01)This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture ... -
Inhibition by rapamycin of ornithine decarboxylase and epithelial cell proliferation in intestinal IEC-6 cells in culture
(East Carolina University, 1997-02)1 Induction of the enzyme ornithine decarboxylase (ODC) appears to be controlled primarily at the level of ODC mRNA translation. The immunosuppressant drug, rapamycin, blocked the induction of ODC in response to serum ... -
Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.
(East Carolina University, 1997-02)3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member ...