Browsing by Author "Neufer, P. Darrell"
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Linking energy state to redox environment through mitochondrial redox circuits
Smith, Cody D (East Carolina University, 2016-11-16)Mitochondria are the primary producers of intracellular H2O2, an oxidant signaling molecule that has gained attention for its role in normal physiological processes and disease etiologies such as skeletal muscle insulin ... -
Metformin Improves Insulin Signaling in Obese Rats via Reduced IKK Action in a Fiber-Type Specific Manner
Bikman, Benjamin Thomas; Zheng, Donghai; Kane, Daniel A.; Anderson, Ethan; Woodlief, Tracey L.; Price, Jesse W.; Dohm, G. Lynis; Neufer, P. Darrell; Cortright, Ronald N. (2010)Metformin is a widely used insulin-sensitizing drug, though its mechanisms are not fully understood. Metformin has been shown to activate AMPK in skeletal muscle; however, its effects on the inhibitor of κB kinaseβ (IKKβ) ... -
Methods for Assessing Mitochondrial Function in Diabetes
Perry, Christopher G.R.; Kane, Daniel A.; Lanza, Ian R.; Neufer, P. Darrell (2013-04)A growing body of research is investigating the potential contribution of mitochondrial function to the etiology of type 2 diabetes. Numerous in vitro, in situ, and in vivo methodologies are available to examine various ... -
Methods for assessing mitochondrial function in diabetes
Perry, Christopher G.R.; Kane, Daniel A.; Lanza, Ian R.; Neufer, P. Darrell (2013-03-14)A growing body of research is investigating the potential contribution of mitochondrial function to the etiology of type 2 diabetes. Numerous in vitro, in situ, and in vivo methodologies are available to examine various ... -
Methods for preventing or treating insulin resistance
Neufer, P. Darrell; Anderson, Ethan; Szeto, Hazel (2009-08-13)The invention provides methods of preventing or treating insulin resistance in a mammalian subject. The methods comprise administering to the subject an effective amount of an aromatic-cationic peptide having at least one ... -
Mitochondrial H₂O₂ emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans
Anderson, Ethan; Lustig, Mary E.; Boyle, Kristen E.; Woodlief, Tracey L.; Kane, Daniel A.; Lin, Chien-Te; Price, Jesse W. III; Kang, Li; Rabinovitch, Peter S.; Szeto, Hazel H.; Houmard, Joseph A.; Cortright, Ronald N.; Wasserman, David H.; Neufer, P. Darrell (2009-03)High dietary fat intake leads to insulin resistance in skeletal muscle, and this represents a major risk factor for type 2 diabetes and cardiovascular disease. Mitochondrial dysfunction and oxidative stress have been ... -
Mitochondrial PE potentiates respiratory enzymes to amplify skeletal muscle aerobic capacity
Heden, Timothy D.; Johnson, Jordan M.; Ferrara, Patrick J.; Eshima, Hiroaki; Verkerke, Anthony R. P.; Wentzler, Edward J.; Siripoksup, Piyarat; Narowski, Tara M.; Coleman, Chanel B.; Lin, Chien-Te; Ryan, Terence E.; Reidy, Paul T.; Castro Brás, Lisandra E. de; Karner, Courtney M.; Burant, Charles F.; Maschek, J. Alan; Cox, James E.; Mashek, Douglas G.; Kardon, Gabrielle; Boudina, Sihem; Zeczycki, Tonya N.; Rutter, Jared; Shaikh, Saame Raza; Vance, Jean E.; Drummond, Micah J.; Neufer, P. Darrell; Funai, Katsuhiko (2019-09-11)Exercise capacity is a strong predictor of all-cause mortality. Skeletal muscle mitochondrial respiratory capacity, its biggest contributor, adapts robustly to changes in energy demands induced by contractile activity. ... -
Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans
Fisher-Wellman, Kelsey H.; Weber, Todd M.; Cathey, Brook L.; Brophy, Patricia; Gilliam, Laura A. A.; Kane, Constance L.; Maples, Jill M.; Gavin, Timothy Patrick; Houmard, Joseph A.; Neufer, P. Darrell (2014-01)Considerable debate exists about whether alterations in mitochondrial respiratory capacity and/or content play a causal role in the development of insulin resistance during obesity. The current study was undertaken to ... -
Mitochondrial Therapy Improves Limb Perfusion and Myopathy Following Hindlimb Ischemia
McClung, Joseph; Ryan, Terence E.; Schmidt, Cameron A.; Alleman, Rick J.; Tsang, Alvin M.; Green, Thomas D.; Neufer, P. Darrell; Brown, David A. (2016-08-01) -
NOVEL MECHANISMS GOVERNING SKELETAL MUSCLE MITOCHONDRIAL BIOENERGETICS : OXPHOS EFFICIENCY AND cAMP/PKA SIGNALING
Lark, Daniel Stephen (East Carolina University, 2014)Understanding the regulation of cellular metabolism is paramount to treating the growing prevalence of metabolic disease worldwide. In cellular metabolism, mitochondrial oxidative phosphorylation (OXPHOS) plays a key role ... -
Novel role for thioredoxin reductase-2 in mitochondrial redox adaptations to obesogenic diet and exercise in heart and skeletal muscle
Fisher-Wellman, Kelsey H.; Mattox, Taylor A.; Thayne, Kathleen; Katunga, Lalage Adalaide; La Favor, Justin D.; Neufer, P. Darrell; Hickner, Robert C.; Wingard, Christopher J.; Anderson, Ethan (2013)Increased fatty acid availability and oxidative stress are physiological consequences of exercise (Ex) and a high-fat, high-sugar (HFHS) diet. Despite these similarities, the global effects of Ex are beneficial, whereas ... -
Overexpression of Long-Chain Acyl-CoA Synthetase 5 Increases Fatty Acid Oxidation and Free Radical Formation While Attenuating Insulin Signaling in Primary Human Skeletal Myotubes
Kwak, Hyo-Bum; Woodlief, Tracey L.; Green, Thomas D.; Cox, Julie H.; Hickner, Robert C.; Neufer, P. Darrell; Cortright, Ronald N. (2019-03-31)In rodent skeletal muscle, acyl-coenzyme A (CoA) synthetase 5 (ACSL-5) is suggested to localize to the mitochondria but its precise function in human skeletal muscle is unknown. The purpose of these studies was to define ... -
PFKFB3-Mediated Glycolysis Rescues Myopathic Outcomes in the Ischemic Limb
McClung, Joseph M.; Ryan, Terence E.; Spangenburg, Espen E.; Schmidt, Cameron A.; Tarpey, Michael D.; Amorese, Adam J.; Yamaguchi, Dean J.; Goldberg, Emma J.; Iñigo, Melissa M.R.; Karnekar, Reema; Brophy, Patricia; Green, Thomas D.; Neufer, P. Darrell; Fisher-Wellman, Kelsey H.; Spangenburg, Espen E. (2020-09-17) -
A single dose of metformin improves whole body insulin sensitivity and alters cellular redox state in skeletal muscle of Zucker fa/fa rats
Lark, Daniel Stephen (East Carolina University, 2010)Energy balance is considered a fundamental requirement of life forms from single cell organisms to higher mammals such as humans. Unfortunately, our species has also discovered the detrimental metabolic responses to excess ... -
Skeletal Muscle Undergoes Fiber Type Metabolic Switch Without Myosin Heavy Chain Switch in Response to Defective Fatty Acid Oxidation
Pereyra, Andrea S.; Lin, Chien-Te; Spangenburg, Espen E.; Neufer, P. Darrell; Fisher-Wellman, Kelsey; Ellis, Jessica M.; Sanchez, Daniela Mesa; Laskin, Julia (2022-02-09) -
Targeted Expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High-Fat Diet-Fed Mice
Spangenburg, Espen; Ryan, Terence E.; Schmidt, Cameron A.; Green, Thomas D.; Neufer, P. Darrell; McClung, Joseph M. (2016-06-09) -
Targeted Expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High-Fat Diet-Fed Mice
McClung, Joseph; Ryan, Terence E.; Schmidt, Cameron A.; Green, Thomas D.; Spangenburg, Espen E.; Neufer, P. Darrell (2016-06-09) -
TIGAR Deficiency Enhances Skeletal Muscle Thermogenesis by Increasing Neuromuscular Junction Cholinergic Signaling
Buddo, Katherine A.; Boykov, Ilya N.; Schmidt, Cameron A.; Lin, Chien-Te; Neufer, P. Darrell (2022-03-07) -
Transcriptional regulation of muscle fatty acid-binding protein.
Carey, Julie O.; Neufer, P. Darrell; Farrar, Roger P.; Veerkamp, Jacques H.; Dohm, G. Lynis (East Carolina University, 1994-03-15)Heart fatty acid-binding protein (H-FABP) is present in a wide variety of tissues but is found in the highest concentration in cardiac and red skeletal muscle. It has been proposed that the expression of H-FABP correlates ... -
Unravelling the mechanisms of menopause-induced insulin resistance: novel roles for estradiol in skeletal muscle mitochondrial function
Torres, Maria Jose (East Carolina University, 2016-11-16)Menopause results in a progressive decline in ovarian production of 17[beta]-estradiol (E2), increased adiposity and a higher risk for type-2 diabetes, but hormone replacement therapies containing E2 reverse most of these ...