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Now showing items 1271-1280 of 1484
Enhancement of Antibodies to the Human Immunodeficiency Virus Type 1 Envelope by Using the Molecular Adjuvant C3d
(East Carolina University, 2003-02)
DNA vaccines expressing the envelope (Env) protein of the human immunodeficiency virus have been relatively ineffective at generating high-titer, long-lasting, neutralizing antibodies in a variety of animal models. In this ...
Identification and Characterization of alcR, a Gene Encoding an AraC-Like Regulator of Alcaligin Siderophore Biosynthesis and Transport in Bordetella pertussis and Bordetella bronchiseptica
(East Carolina University, 1998-02)
A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin ...
Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.
(East Carolina University, 1996-08)
Bordetella bronchiseptica mutants BRM1, BRM6, and BRM9 fail to produce the native dihydroxamate siderophore alcaligin. A 4.5-kb BamHI-SmaI Bordetella pertussis genomic DNA fragment carried multiple genes required to restore ...
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa
(East Carolina University, 2002-12)
A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in ...
Transcriptional Activation of Bordetella Alcaligin Siderophore Genes Requires the AlcR Regulator with Alcaligin as Inducer
(East Carolina University, 2001-01)
Genetic and biochemical studies have established that Fur and iron mediate repression of Bordetella alcaligin siderophore system (alc) genes under iron-replete nutritional growth conditions. In this study, transcriptional ...
Transcriptional Analysis of the Bordetella Alcaligin Siderophore Biosynthesis Operon
(East Carolina University, 1998-02)
The alc gene cluster of Bordetella pertussis includes three genes, alcA, alcB, and alcC, which are involved in alcaligin siderophore biosynthesis in response to iron starvation. The production of AlcA, AlcB, and AlcC in ...
Enhanced Avidity Maturation of Antibody to Human Immunodeficiency Virus Envelope: DNA Vaccination with gp120-C3d Fusion Proteins
(East Carolina University, 2001-06-10)
DNA vaccination can elicit both humoral and cellular immune responses and can confer protection against several pathogens. However, DNA vaccines expressing the envelope (Env) protein of human immunodeficiency virus (HIV) ...
Endometrial carcinoma in vitro chemosensitivity testing of single and combination chemotherapy regimens using the novel microculture kinetic apoptosis assay: implications for endometrial cancer treatment
(East Carolina University, 2010-03)
Objective: The in vitro microculture kinetic (MiCK) apoptosis assay has been used to predict single or combination chemotherapy response in leukemia patients. This feasibility study addressed MiCK in endometrial cancer ...
RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst
(East Carolina University, 2006-07)
Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in ...
Effects of palindromes on in vivo DNA replication and mutagenesis in bacteriophage phi X174 RF DNA.
(East Carolina University, 1988-11-25)
Bacteriophage IX174 mutants with insertions of palindromic DNA sequences are rapidly outgrown by competing wild-type phage (Muller & Turnage, J. Mol. Biol. 189: 285). The basis for this defect was investigated and found ...