Search
Now showing items 71-80 of 82
Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.
(East Carolina University, 1997-02)
3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member ...
Equilibration and Exchange of Fluorescently Labeled Molecules in Skeletal Muscle Fibers Studied Using Confocal Microscopy
(East Carolina University, 1995-04)
Diffusion of molecules into skinned muscle fibers is often necessary when studying muscle contraction and its regulation. Usually, it is assumed that diffusion of molecules is fast also in the structured system of a muscle ...
Regulation of lipoprotein lipase mRNA content in 3T3-L1 cells by tumour necrosis factor.
(East Carolina University, 1988-02-01)
Tumour necrosis factor (TNF) was previously shown to suppress lipoprotein lipase (LPL) synthesis and activity in 3T3-L1 adipocytes. The present study examined the effect ofTNF on amounts ofmRNA for LPL in 3T3-Ll cells. ...
Insulin responsiveness in skeletal muscle is determined by glucose transporter (Glut4) protein level.
(East Carolina University, 1990-09-01)
Glucose transport in skeletal muscle is mediated by two distinct transporter isoforms, designated muscle/adipose glucose transporter (Glut4) and erythrocyte/HepG2/brain glucose transporter (Glutl), which differ in both ...
HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype
(East Carolina University, 2009-05-29)
In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements in the 3?- untranslated region. HuR may influence expression of its ligand mRNA through regulation of polyadenylation, translocation of the ...
The Interaction of Caldesmon with the COOH Terminus of Actin
(East Carolina University, 1991-10-25)
Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon ...
The RXR-alpha C-terminus T462 is a NMR sensor for coactivator peptide binding
(East Carolina University, 2008-02-22)
The C-terminal activation function-2 (AF-2) helix plays a crucial role in retinoid X receptor alpha (RXRα)-mediated gene expression. Here, we report a nuclear magnetic resonance (NMR) study of the RXRα ligand-binding domain ...
A germline-specific isoform of eIF4E (IFE-1) is required for efficient translation of stored mRNAs and maturation of both oocytes and sperm
(East Carolina University, 2009-05-15)
Fertility and embryonic viability are measures of efficient germ cell growth and development. During oogenesis and spermatogenesis, new proteins are required for both mitotic expansion and differentiation. Qualitative and ...
Negative Charges at Protein Kinase C Sites of Troponin I Stabilize the Inactive State of Actin
(East Carolina University, 2008-01-15)
Alterations in the troponin complex can lead to increases or decreases in contractile activity. Most mutations of
troponin that cause hypertrophic cardiomyopathy increase the activity of cardiac muscle fibers. In at least ...
Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity
(East Carolina University, 1993-01-05)
A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. The deoxyguanosine-resistant cells (dGuo-R) were 126-fold less sensitive to deoxyguanosine than the wild-type cells. The IC50 values for araC ...