Browsing Biochemistry and Molecular Biology by Title
Now showing items 33-52 of 82
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HUMAN RNA METHYLTRANSFERASE METTL16 AFFECTS MULTIPLE CELL PROCESSES THROUGH DIFFERENTIAL FUNCTION OF ITS DOMAINS
(East Carolina University, 2022-11-10)ABSTRACT In recent years, disease therapies have been developed to target specific RNA and protein expression changes in the affected cells/tissue. The RNA post-transcriptional modification, methyl-6-adenosine (m6A), has ... -
HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes
(East Carolina University, 2007-03-30)HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA, HuR is recognized by adapter proteins which then facilitate nuclear export of the ... -
HuR involvement in mitotic clonal expansion during acquisition of the adipocyte phenotype
(East Carolina University, 2009-05-29)In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements in the 3?- untranslated region. HuR may influence expression of its ligand mRNA through regulation of polyadenylation, translocation of the ... -
Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1
(East Carolina University, 2003-06-01)The p21-activated protein kinases (PAKs) have been implicated in cytoskeletal rearrangements and modulation of non-muscle contractility. Little, however, is known about the role of the PAK family members in smooth muscle ... -
Insulin responsiveness in skeletal muscle is determined by glucose transporter (Glut4) protein level.
(East Carolina University, 1990-09-01)Glucose transport in skeletal muscle is mediated by two distinct transporter isoforms, designated muscle/adipose glucose transporter (Glut4) and erythrocyte/HepG2/brain glucose transporter (Glutl), which differ in both ... -
The Interaction of Caldesmon with the COOH Terminus of Actin
(East Carolina University, 1991-10-25)Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon ... -
The involvement of cap-independent mRNA translation in cell fate decisions
(East Carolina University, 2014)During cell stress many biochemical processes are shut down. For example, global mRNA translation initiation is inhibited due to the disruption of the cap-dependent mRNA recruitment mechanism. One specific example of ... -
Kinetic and Thermodynamic Contributions to an Intermolecular Mechanism of Subunit Communication: Coordination of Pyruvate Carboxylase Activity Among Spatially Distinct Active Sites
(East Carolina University, 2016-07-13)Catalysis occurring in a multifunctional enzyme at spatially distinct active sites is controlled by an array of factors, including the structure of the enzyme, ligand binding, and productive interaction of substrates to ... -
Kinetics of Binding of Caldesmon to Actin
(East Carolina University, 1995-04-28)The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ... -
Lipid mediators of insulin resistance: ceramide signalling down-regulates GLUT4 gene transcription in 3T3-L1 adipocytes.
(East Carolina University, 1996-10-01)We have previously demonstrated that chronic exposure of 3T3- L1 adipocytes to tumour necrosis factor-α (TNF) resulted in a marked decrease (~90%) in cellular GLUT4 (insulin-responsive glucose transporter) mRNA content as ... -
Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity
(East Carolina University, 1992-08-15)Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several ... -
A Long Helix from the Central Region of Smooth Muscle Caldesmon
(East Carolina University, 1991-07-25)The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic properties and the electron ... -
Low adenosine anti-sense oligonucleotide agent, composition, kit and treatments
(2000-02-24)A composition comprises a nucleic acid comprising an oligo anti-sense to a target such as polypeptide(s) associated with an ailment afflicting lung airways, genes and mRNAs encoding them, genomic and mRNA flanking regions, ... -
Measles vaccine vectors and methods of use
(2003-03-20)The present invention provides a novel vaccine expressing a fusion of the measles hemagglutinin (H) protein and the complement component, C3d, to enhance the titers of neutralizing antibody. Plasmids were generated that ... -
Method for the synthesis of 3-substituted indolizine and benzoindolizine compounds
(2006-07-13)A method of making a compound of Formula (I) comprises reacting a compound of Formula (II) with a compound such as R1OH or R1SH, to produce said compound of Formula (I). Compounds of Formula (I) are useful, among other ... -
Molecular mechanisms of TMEFF2 action in Prostate Cancer
(East Carolina University, 2013)The transmembrane protein with epidermal growth factor and two follistatin motifs 2 (TMEFF2) is an evolutionarily conserved type I transmembrane protein expressed in the embryo and limited adult tissues, mainly the brain ...